PCR-Southern印迹杂交,PCR-Southern blots analysis
Southern blotSouthern印迹杂交
1.Southern blotting analysis of telomeric repetitive DNA sequences in Giardia canis;犬贾第虫端粒重复序列的Southern印迹杂交分析
2.Objective To confirm the integration of transduced genes in the genomic DNA of host cells bySouthern blot analysis.目的应用Southern印迹杂交法 (Southernblot)证实转移基因整合至宿主细胞基因组DNA。
3)PCR-Southern blottingPCR-Southern杂交
1.The results showed that two special fragments of about 460 bp and 690 bp were obtained,andPCR-Southern blotting about the.用抗根结线虫基因设计的特异引物进行扩增最终获得了1条大约460 bp大小的特异片段;用抗胞囊线虫基因设计的特异引物进行扩增最终获得了1条大约690 bp大小的特异片段,进一步进行了PCR-Southern杂交,确认了该2条特异引物的真实性。
4)RT-PCR-Southern blotRT-PCR-Southern杂交
5)Southen and Northen blottingSouthern和Northern印迹杂交技术
6)PCR-Southern hybridizationPCR-Southern分子杂交
延伸阅读
PC 聚碳酸酯(双酚A型)化学名称2，2-双(4-羟基苯基)丙烷聚碳酸酯，是由酯交换法和光气化法制得。平均分子量3.57×104。无定形透明颗粒．无味、天臭、无毒。相对密度1．20-1.43。没有明显熔点，220-230℃呈熔融状态。透光率高，吸水性低，耐冲击，韧性好．蠕变小，制品尺寸稳定。耐热性和耐寒性较好．玻璃化温度149-150℃．热变形温度135℃(1．82MPa)、分解温度＞310 ℃，脆性温度－l00 ℃，使用温度-100～120 ℃。耐稀酸、耐油、不耐碱。溶于二氯甲烷、二氯乙烷、氯仿、三氯乙烯、四氯乙烷、四氢呋喃、三甲酚、磷酸三甲酯等。疲劳强度较低，容易产生应力开裂。具有优良的高温电性能，具有耐燃自熄性。拉伸强度 ＞60MPa, 伸长率＞60％，弯曲强度＞95MPa。
