PCR-Southern杂交,PCR-Southern blotting
1)PCR-Southern blottingPCR-Southern杂交
1.The results showed that two special fragments of about 460 bp and 690 bp were obtained,andPCR-Southern blotting about the.用抗根结线虫基因设计的特异引物进行扩增最终获得了1条大约460 bp大小的特异片段;用抗胞囊线虫基因设计的特异引物进行扩增最终获得了1条大约690 bp大小的特异片段,进一步进行了PCR-Southern杂交,确认了该2条特异引物的真实性。
2)RT-PCR-Southern blotRT-PCR-Southern杂交
3)PCR-Southern hybridizationPCR-Southern分子杂交
4)PCR-Southern blots analysisPCR-Southern印迹杂交
1.Through PCR analysis,the agarose gel electrophoresis result displayed that six of eight Kanamycin fastness seedlings had target genes;ThroughPCR-Southern blots analysis,the result showed that the positive rate was 50%,and further indicated that the target genes had been inserted into the genomes of Populus nigra×Populus deltoids "108" plants successfully.通过PCR检测,凝胶电泳结果显示8个卡那霉素抗性芽中,6个存在目的基因;经PCR-Southern印迹杂交检测,阳性率为50%,进一步表明目的基因成功整合到欧美杨108号基因组内。
5)Southern blotSouthern杂交
1.Using RT-PCR/Southern blot to detect the change of Brn-4 mRNA expression in rat hippocampus after fimbria fornix transection;RT-PCR/Southern杂交法检测穹窿海马伞切割大鼠海马Brn-4 mRNA的表达变化
2.Methods 105 peripheral blood samples were collected from healthy individuals of different ages,ranged from 0 to 81 years old,and the terminal restriction fragment (TRF) length was measured bySouthern blot analysis.方法抽取西藏那曲地区0~81岁健康人外周血样本105例,其中男性53例,女性52例,采用Southern杂交法检测其端粒限制性片断平均长度。
3.Methods Using RT PCR/Southern blot to detect the ALB mRNA in the peripheral blood from 29 patients with hepatocellular carcinoma (HCC) and 41 cases without HCC (8 cases of cirrhosis, 4 cases of acute hepatitis, 15 cases of chronic hepatitis, 2 cases of hepatic angioma, 2 cases of non HCC hepatic tumor and 10 normal voluteers).方法分离原发性肝癌患者以及肝硬化、急慢性肝炎、肝转移癌、肝脏良性肿瘤患者和健康志愿者的外周血单个核细胞 ,提取单个核细胞的总RNA ,进行RT PCR ,将RT PCR产物再进行Southern杂交。
英文短句/例句
1.Southern Blot and Expression Analysis of 4 α-galactosidases in Cucumber (Cucumis Sativus L.);黄瓜4种α-半乳糖苷酶的Southern杂交及表达分析
2.Technological Improvement of Southern Blot Using Digoxigenin-labeled Probes with Random-primed Method地高辛随机引物法标记探针的Southern杂交技术优化
3.Studied on DIG-labeled Southern Blot Analysis and Cold Resistance Correlation Characteristics in Transgenic Lines of Apple Carrying Exogenous CBF Gene转CBF基因苹果株系地高辛标记Southern杂交分析及相关抗寒性状研究
4.A Novel Classification Patterns of Cellulases and New Tool of Analyzing Southern Blotting Based on Bioinformatics Approach;用生物信息学方法探索纤维素酶分类模式和设计分析Southern杂交的工具
5.dna rna hybridationdna rna 杂交
6.Southern blottingsouthern印迹法，DNA印迹法
7.crossing and heterosis of animal杂交和杂种优势（家畜）
8.a filial generation【生】杂交后代, 子代
9.The hybrid offspring of a zebra and a horse.杂交斑马斑马与马杂交的后代
10.Improvements of 8-node Biquadratic Q8-element by Hybrid/Combined Hybrid FEMS;杂交/组合杂交方法对Q8-元改进
11.Lily Distant Hybridization and Seed Characteristics百合杂交育种及杂交种子特性的研究
12.Further refinements of pollen management include controlled crosses where the pedigree of the cross is required.更细致的花粉管理工作，包括控制杂交，即必要杂交的系谱杂交。
13.The noise of the traffic worried her.交通的嘈杂声使他不安。
14.a hybrid animal, plant由杂交生成的动物、 植物.
15.miscellaneous offence [traffic]杂项违例事件〔交通〕
16.Somatic cell hybridization is technique.体细胞杂交是一种技术。
17.an inferior dog or one of mixed breed.一种低劣的或杂交的狗。
18.Of mixed breed; not thoroughbred.劣种的杂交的，非纯种的
相关短句/例句
RT-PCR-Southern blotRT-PCR-Southern杂交
3)PCR-Southern hybridizationPCR-Southern分子杂交
4)PCR-Southern blots analysisPCR-Southern印迹杂交
1.Through PCR analysis,the agarose gel electrophoresis result displayed that six of eight Kanamycin fastness seedlings had target genes;ThroughPCR-Southern blots analysis,the result showed that the positive rate was 50%,and further indicated that the target genes had been inserted into the genomes of Populus nigra×Populus deltoids "108" plants successfully.通过PCR检测,凝胶电泳结果显示8个卡那霉素抗性芽中,6个存在目的基因;经PCR-Southern印迹杂交检测,阳性率为50%,进一步表明目的基因成功整合到欧美杨108号基因组内。
5)Southern blotSouthern杂交
1.Using RT-PCR/Southern blot to detect the change of Brn-4 mRNA expression in rat hippocampus after fimbria fornix transection;RT-PCR/Southern杂交法检测穹窿海马伞切割大鼠海马Brn-4 mRNA的表达变化
2.Methods 105 peripheral blood samples were collected from healthy individuals of different ages,ranged from 0 to 81 years old,and the terminal restriction fragment (TRF) length was measured bySouthern blot analysis.方法抽取西藏那曲地区0~81岁健康人外周血样本105例,其中男性53例,女性52例,采用Southern杂交法检测其端粒限制性片断平均长度。
3.Methods Using RT PCR/Southern blot to detect the ALB mRNA in the peripheral blood from 29 patients with hepatocellular carcinoma (HCC) and 41 cases without HCC (8 cases of cirrhosis, 4 cases of acute hepatitis, 15 cases of chronic hepatitis, 2 cases of hepatic angioma, 2 cases of non HCC hepatic tumor and 10 normal voluteers).方法分离原发性肝癌患者以及肝硬化、急慢性肝炎、肝转移癌、肝脏良性肿瘤患者和健康志愿者的外周血单个核细胞 ,提取单个核细胞的总RNA ,进行RT PCR ,将RT PCR产物再进行Southern杂交。
6)Southern hybridizationSouthern杂交
1.Digested with restriction endonuclease, of chromosomal DNA was performed bySouthern hybridization with pro.对染色体 DNA进行限制性酶切 ,以第一类整合酶基因 int I1( DIG标记 )为探针 ,进行 Southern杂交。
2.The result ofSouthern hybridization demonstrated that the differential fragments were true.Southern杂交结果证明了差异片段的真实性。
3.Digesting the constructs and making southern hybridization with the cDNA of rat 20 αHSD labeled with DIG,we revealed the 4.0kb的DNA大片段 ,将此片段与载体质粒连接得到重组质粒 酶切重组质粒后电泳 ,再以地高辛标记的 2 0αHSD基因的cDNA为探针进行Southern杂交 实验获得了大片段的重组质
延伸阅读
immune PCR分子式：CAS号：性质： 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上，从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上，进而证明有抗原存在。目前最为敏感的检测方法，理论上可测得一个抗原分子的存在。
