斑点杂交,Dot blot hybridization
1)Dot blot hybridization斑点杂交
1.Detection of IHHNV in shrimp by dot blot hybridization with digoxigenin labeled DNA probe by PCR;PCR法制备地高辛标记DNA探针斑点杂交检测对虾传染性皮下及造血组织坏死病毒(IHHNV)
2.anguillarum by dot blot hybridization.选取2株鳗弧菌和8株与鳗弧菌亲缘关系非常接近的常见水产动物致病弧菌,通过斑点杂交法对制备的探针进行特异性检测,结果显示该探针对鳗弧菌杂交阳性,而与弧菌属的其它8株细菌均无交叉反应。
3.Then white spot syndrome virus was detected by PCR and dot blot hybridization before and after soaking Brachionus plicatilis.对褶皱臂尾轮虫进行白斑综合征病毒(WSSV)攻毒,然后将攻毒前后的轮虫进行PCR及核酸探针斑点杂交检测。
英文短句/例句
1.Detection of Jaagsiekte Sheep Retrovirus by Dot blot hybridization绵羊肺腺瘤病毒的斑点杂交技术检测
2.Dot-blot hybridization for the detection of aspergillus fumigatus infection斑点杂交法用于检测烟曲霉菌感染的实验研究
3.Rapid Detection and Identification of Mycobacterium Species by Amplification of rpoB Squences and Reverse Dot Blot Hybridization Assay;rpoB基因-PCR-反向斑点杂交快速鉴定分枝杆菌菌种的研究
4.Detection of Nucleic Acid of Poultry Virus with Nucleic Acid Probe and Molecule Dot Blot Hybridization Technique;用核酸探针和分子斑点杂交技术检测家禽病毒的核酸
5.Dot-blot Hybridization for Detection of Cucurbit Viruses by Digoxigenin-Labelled cDNA Probes;地高辛标记探针检测五种葫芦科作物病毒的斑点杂交方法
6.Cloning and Sequencing of MOMP Gene of Salpingitis Chlamydial in Hens and Detection with Dot Blot Hybridization;鸡输卵管囊肿衣原体MOMP基因克隆、序列测定及斑点杂交检测
7.A PCR Based Reverse-Line Blot Hybridization Method for Rapid Detection of RpoB Mutations in Isolates of Mycobacterium Tuberculosis;反相斑点杂交快速检测结核分枝杆菌rpoB基因突变
8.SLC25A13 Gene Mutation Analyzed by PCR-reverse Dot-blotting Hybridization MethodPCR-反向斑点杂交技术在SLC25A13基因突变检测中的应用研究
9.The Experimental and Clinical Study of Dot-blot Hybridization in Diagnosis of Invasive Aspergillosis斑点杂交法在侵袭性烟曲霉菌感染诊断中的实验及临床研究
10.Development of a Reverse Dot Blot System for Rapid Detection of HBV Genotypes反向斑点杂交法快速检测HBV基因型反应条件的优化和建立
11.Genotyping of Influenza Viruses by Reverse Dot Blot Method反向斑点杂交检测甲/乙型流感病毒及其基因分型的方法研究
12.Study on diagnosis of invasive aspergiuosis by PCR and dot blot hybridizationPCR结合反向斑点杂交法检测侵袭性曲霉感染的初步实验研究
13.An improved probe labeling and dot-blot hybridization assay for human rotavirus VP7 genotyping改进探针标记法斑点杂交在轮状病毒VP7分型中的应用
14.Epidemiological investigation of frog leptospirosis by dot blotting斑点杂交技术用于蛙钩端螺旋体病的流行病学调查研究
15.Detection of the tlh Gene of Vibrio Parahaemolyticus by Dot-blot HybridizationPCR法制备地高辛标记DNA探针斑点杂交检测副溶血性弧菌tlh基因
16.Application and Evaluation of Reverse Dot Blot (RDB) Hybridization Assay for HBV YMDD Motif Mutants反向斑点杂交技术检测HBV YMDD基序变异的应用和评价
parison of the enzyme dot hybridization assay with double antibody sandwich assay in the detection of Hantavirus infection酶斑点杂交法与双抗体夹心法检测汉坦病毒感染的比较
18.Detection and identification of Aspergillus species from formalin-fixed,paraffin-embedded human tissues by PCR and dot blot hybridizationPCR结合反向斑点杂交法检测石蜡包埋组织中的曲霉感染
相关短句/例句
Dot Blot斑点杂交
1.Evaluation or Polymerase Chain Reaction and Dot blot for HBV DNA Detection;聚合酶链反应结合斑点杂交测定乙型肝炎病毒DNA的评价
2.A quantitative dot blot method of detection for target gene relatively copy numbers in DNA sample;DNA样品目的基因相对拷贝数的定量斑点杂交测定方法
3.Methods Dot blot of chromatosome and plasmid DNA were performed in 2 Enterobacter cloacaes.方法分别提取细菌的染色体DNA和质粒DNA通过斑点杂交的方式 ,并将整合子聚合酶链反应(PCR)产物测序 ,来确定编码AmpC酶DHA 1型基因存在的位置。
3)dot-blot hybridization斑点杂交
1.DNA signatures of Coxiella burnetti analyzed by PCR and DNAdot-blot hybridization;PCR和DNA斑点杂交分析贝氏柯克斯体的核酸标识
2.The detection sensitivities ofdot-blot hybridization for E.本研究合成了苹果茎沟病毒(ASGV)、苹果褪绿叶斑病毒(ACLSV)和苹果茎痘病毒(ASPV)的生物素标记cDNA探针,对斑点杂交和免疫印迹杂交检测这3种病毒的效果进行了分析。
3.To identify the features of DPV CHv dUTPase gene,a large number of bioinformatics analysis tools and the RNAdot-blot hybridization technique were used.本文应用大量生物信息学分析工具以及 RNA斑点杂交技术对本实验室在构建鸭瘟病毒(Duck plague virus,DPV)CHv 株基因文库时新发现的 dUTPase 基因(GenBank 登录号 DQ486149)进行了分子特性及转录时相分析。
4)dot hybridization斑点杂交
1.Detection of -Sugarcane mosaic virus- bydot hybridization and RT PCR technique;利用斑点杂交法和RT-PCR技术检测甘蔗花叶病毒
2.(Methods The expressions) of IGF-ⅠmRNA of WI-38 and Hela cell lines in silver nanomaterial group, rare-earth nanomaterial group and control group were tested by in situ hybridization,dot hybridization and quantitative analysis technique.②方法通过原位杂交、斑点杂交及定量分析技术分别检测银系纳米材料组、稀土纳米材料组与对照组WI-38及Hela细胞IGF-ⅠmRNA表达。
3.The positive clones were selected bydot hybridization with Dig-labeled probe.利用地高辛标记探针进行斑点杂交检测 ,筛选出阳性克隆 ,拟用该克隆转化马铃薯细胞 ,研制新型轮状病毒疫苗。
5)dot blotting斑点杂交
1.Thedot blotting detection method was established to detect the V.分别用胶原蛋白酶DNA探针和外膜蛋白DNA探针对实验室保种的溶藻弧菌以及鱼排分离的溶藻弧菌进行斑点杂交检测,检测结果表明,胶原蛋白酶基因探针与保种的溶藻弧菌以及鱼排分离的溶藻弧菌DNA产生较强的阳性反应,而与霍乱弧菌、哈氏弧菌和副溶血弧菌DNA等均无反应,表明该胶原蛋白酶DNA探针的特异性较强;而外膜蛋白基因探针与保种的溶藻弧菌以及鱼排分离的溶藻弧菌DNA产生较强的阳性反应,但与哈氏弧菌和副溶血弧菌DNA也出现了较强的阳性反应,与霍乱弧菌DNA则无反应,表明该外膜蛋白DNA探针的特异性较低。
6)RNA dot blotRNA斑点杂交
1.The expression of hTR and telomerase activity in gastric mucosa was measured byRNA dot blot and telomere repeat amplification protocol (TRAP) assay , respectively.方法制备人端粒酶RNA ,(humantelomeraseRNA ,hTR)的cDNA探针 ,分别应用RNA斑点杂交与端粒重复序列扩增法 (TRAP)分析检测不同胃粘膜的端粒酶RNA的表达与端粒酶活性。
延伸阅读
斑点在一种颜色的物体表面上显露出来的别种颜色的点子。
