Western blot技术,Western blot methods
Western-blotWestern-blot技术
1.Methods:RT-PCR andWestern-blot were used to identify the expression of LRIG2 mRNA and protein in 38 bladder cancer tissue and 16 normal bladder epithelium tissue.方法:采用逆转录-聚合酶链反应和Western-blot技术检测了38例膀胱癌组织和16例正常膀胱黏膜组织中LRIG2mRNA和蛋白的表达情况。
3)western blotWestern-Blot
4)Western blotWestern blot法
1.The expression of p-JNK,caspase-3 and PARP was detected byWestern blots.方法:采用MTT法观察不同浓度的As2O3对人类肝癌细胞株HepG2细胞生长的抑制作用;以流式细胞术观察细胞的凋亡率;以Western blot法检测JNK、p-JNK、Caspase-3及PARP蛋白在As2O3作用下及SP600125阻断JNK信号转导通路情况下的表达。
5)Western-blotWestern-blot法
1.The protein levels of SOCS-3 were further analysed byWestern-blot.方法实时荧光定量PCR法检测60例脑梗死患者和40例健康对照者的PBMC中SOCS-3 mRNA表达,Western-blot法检测其蛋白质水平的表达。
2.Methods:An immunohistochemical analysis with antibodies to eIF4E was performed on Fifty-two specimens of the LSCC,Twenty specimens of dysplasia,Thirty specimens of hyperplasia and ten specimens of normal mucosa;Western-blot analysis was performed on tumor core,transition core,tumor-free core of twenty specimens of fresh LSCC using anti-eIF4E,antiserums.方法:采用免疫组织化学法检测52例蜡块LSCC组织、20例喉黏膜不典型增生组织、30例喉良性增生组织及10例正常喉黏膜组织中eIF4E基因蛋白的表达情况;用Western-blot法检测20例新鲜LSCC标本的癌中心区(TC)、过渡区(TR)及病理证实的无癌区(TF)中eIF4E的表达情况。
3.Material 40 cases of tissues of gastric cancer and 40 cases of normal tissues were collected, in accordance withβ-actin, and the expression of TSC1 protein were detected by Western-Blot.方法收集胃癌组织标本40例，癌旁正常组织标本40例，用Western-Blot法检测样本中TSC1蛋白和β-actin蛋白含量，分别测定TSC1带与β-actin带的黑度值，以黑度值的比值A值表示TSC1蛋白表达水平。
6)Western Blot-ELSIAWestern Blot-ELISA
延伸阅读
We分子式：CAS号：性质：用来研究流体力学的一个准数，其表示式为。式中，ρ为流体密度，kg/m3；u为流体速度，m/s；L为定性长度，m；σ为表面张力，N/m。韦伯数的物理意义为惯性力与表面张力之比。
