Southern印迹,Southern blotting
1)Southern blottingSouthern印迹
1.Methods PFGE(pulsed field gradient gel electrophoresis)combined withSouthern blotting hybridization was used to determine the sizes of DMs in human ovarian cancer cells UACC-1598 and MTX-resistant cell derived from mouse fibroblasts 3T3R500.方法应用PFGE(Pulsed field gradient gel electrophoresis)与Southern印迹杂交技术,对人卵巢癌细胞UACC-1598及氨甲蝶呤(Methotrexate,MTX)抗性的小鼠胚胎成纤维细胞中DMs的大小进行检测。
2.Methods: Telomere length was examined bySouthern blotting by using labelled oligonucleotide(TTAGGG)3 probe with digoxin.方法:以地高辛标记寡核苷酸(TTAGGG)3为探针,对基因组DNA进行Southern印迹检测,经CDP-Star显色后与DNA标准分子量比较,利用图像分析系统计算端粒长度。
英文短句/例句
1.chromosome blotting染色体印迹[通过脉冲电场凝胶电泳分离染色体DNA并进行Southern印迹]
2.Southern blottingsouthern印迹法，DNA印迹法
3.electroblotting device电印迹仪，电印迹装置
4.Northern blottingNorthern印迹（法），RNA印迹（法）
5.Northwestern blottingNorthwestern印迹（法），RNA-蛋白质印迹（法）
6.southwestern blottingsouthwestern印迹法，DNA-蛋白质印迹法
7.A mark or pattern produced by imprinting.压印产生的痕迹或图案
8.(of handwriting, print, etc.) not legible.（关于笔迹，印迹，等）不清晰的。
9.(of handwriting, print, etc.) able to be read.（关于笔迹，印迹，等）能够被阅读的。
10.an impression taken from an engraved steel plate.从钢制雕刻板上印出来的印迹。
11.protoBlot system[商]蛋白印迹系统[Promega公司生产的蛋白质印迹成套试剂]
12.Loss of Imprinting of IGF2 Gene in Endometrium of Assisted Reproductive Technology Patients印迹基因IGF2在辅助生殖患者子宫内膜中印迹丢失及意义
13.Preparation of Molecular Surface Imprinting Material and Its Identification Properties for Bilirubin表面印迹法制备胆红素分子印迹材料及其识别性能
14.Synthesis and Properties Study of Mercury Ion Imprinted Polymer双重印迹法制备汞离子印迹聚合物及其性能研究
15.Preparation and recognition properties of GA_3-imprinted polymers/membranes赤霉酸分子印迹聚合物及印迹膜的制备与性能研究
16.A mark produced on a surface by pressure.印记，印痕由于压力而在表面上形成的痕迹
17.Southwestern blot hybridization of DN A-binding proteinsDNA结合蛋白的Southwestern印迹杂交
18.The tracks of the horse"s hoofs were seen deeply marked in the dirt.马蹄的痕迹仍然深深地印在泥土上。
相关短句/例句
Southern blotSouthern印迹
1.Methods The length of D4Z4 repeats on chromosome 4q35 in 191 normal individuals in Shanghai was determined by pulsed-field gel electrophoresis andSouthern blotting after double digestion with EcoRⅠ and BlnⅠ.方法191名正常上海人的基因组DNA经EcoRⅠ/B1nⅠ双酶水解后,应用脉冲场凝胶电泳及Southern印迹测定其染色体4q35位点的D4Z4片段长度,并对短的D4Z4片段用KpnⅠ酶进行部分酶切以计数其D4Z4串联重复序列数。
2.Methods Densitometry of the bands onSouthern blot, polymerase.方法用Southern印迹方法检测 7例 CPEO和 4例 KSS患者的肌肉组织 mt DNA,并进一步用聚合酶链反应产物直接测序来明确缺失的具体范围 ;用聚合酶链反应 -限制性内切酶分析法检测有无 mt D-NA A32 4 3G点突变。
3.A simple,rapid,sensitive,low-cost and reproducible protocol ofSouthern blot analysis was developed based on a comprehensive analysis of the previous reports.在综合前人方法的基础上,改良了一种简捷、灵敏、廉价、效果可靠的Southern印迹杂交方案,使用0。
3)Southern blotSouthern印迹法
4)Southern blotSouthern印迹杂交
1.Southern blotting analysis of telomeric repetitive DNA sequences in Giardia canis;犬贾第虫端粒重复序列的Southern印迹杂交分析
2.Objective To confirm the integration of transduced genes in the genomic DNA of host cells bySouthern blot analysis.目的应用Southern印迹杂交法 (Southernblot)证实转移基因整合至宿主细胞基因组DNA。
5)southern blot analysiSouthern印迹分析
6)PCR-Southern blots analysisPCR-Southern印迹杂交
1.Through PCR analysis,the agarose gel electrophoresis result displayed that six of eight Kanamycin fastness seedlings had target genes;ThroughPCR-Southern blots analysis,the result showed that the positive rate was 50%,and further indicated that the target genes had been inserted into the genomes of Populus nigra×Populus deltoids "108" plants successfully.通过PCR检测,凝胶电泳结果显示8个卡那霉素抗性芽中,6个存在目的基因;经PCR-Southern印迹杂交检测,阳性率为50%,进一步表明目的基因成功整合到欧美杨108号基因组内。
延伸阅读
“诺森”印迹法分子式：CAS号：性质：又称RNA印迹（法）。与DNA斑迹法过程类似的技术。用于从凝胶中转移出RNA片段转移至硝酸纤维素膜，尼龙膜等，并用探针进行杂交（称“北方”杂交）以显示目的区段的位置。显示RNA的大小及丰度。
