蛋白印迹,Western blot
1)Western blot蛋白印迹
1.Methods The immunohistochemistry SABC method andWestern blot were used to determine the expression of Annexin A1 in 120 cases of normal breast tissues,ductal hyperplasia,non-invasive carcinoma and invasive carcinoma.方法应用免疫组织化学SABC方法和蛋白印迹检测乳腺正常组织、良性病变(纤维腺瘤)、癌前病变(乳腺囊性增生症)、非浸润性导管癌、浸润性导管癌中Annexin A1的表达情况。
2.The aliquot of proteins was subjected to SDS-PAGE,followed byWestern blot analysis in which individual sera were tested for primary antibody.用肺癌患者及健康对照者的血清作第一抗体进行蛋白印迹检测。
3.Methods The immunohistochemical method andWestern blot method were used to determine expression of COX-2 protein in specimens of 47 cases(33 cases by IHC and 14 by Western Blot)of nasopharyngeal carcinoma and 24 cases of normal nasopharyngitis tissues.方法分别应用免疫组化方法(IHC)和蛋白印迹方法(Western Blot)检测47例鼻咽癌组织(其中用IHC33例,Western Blot14例)及24例鼻咽部炎症组织中COX-2蛋白的表达。
英文短句/例句
1.protoBlot system[商]蛋白印迹系统[Promega公司生产的蛋白质印迹成套试剂]
2.Preparation of monodisperse imprinting microspheres for Serum Albumin新型单分散血清白蛋白印迹微球的制备
3.③The changes of BMP2 and osteopontin were detected by immunohistochemistry and Western blotting.③骨形态发生蛋白2与骨桥蛋白检测:采用免疫组织化学染色与蛋白印迹方法。
4.Biological Factors Affecting the Determination of HIV Antibody影响蛋白印迹法检测HIV抗体结果的生物学因素分析
5.Application of autoblot system in detecting ENA polypeplide autoantibody profile自动蛋白印迹仪在检测抗可提取核抗原多肽抗体谱中的应用
6.Northwestern blottingNorthwestern印迹（法），RNA-蛋白质印迹（法）
7.southwestern blottingsouthwestern印迹法，DNA-蛋白质印迹法
8.Southwestern blot hybridization of DN A-binding proteinsDNA结合蛋白的Southwestern印迹杂交
9.Study on Protein－Imprinted Soft-wet Gel Polymeric Beads;蛋白质印迹软湿凝胶聚合物微球的研究
10.Bio-imprinting of Protein/DNA Macromolecules and Electro-driven Catalysis of Enzyme;蛋白质/核酸的生物印迹及酶的电驱动催化研究
11.Preparation and Selective Permeation of Protein Molecularly Imprinted Membranes蛋白质分子印迹膜的制备和渗透性研究
12.Studies on Chitosan Coated on Silica for Imprinting Bovine Hemoglobin壳聚糖包裹硅胶载体印迹牛血红蛋白的研究
13.Detection of E-cadherin expressions in lung cancer tissues by Western blot analysis蛋白质免疫印迹技术检测肺癌组织上皮钙粘蛋白的表达
14.Optimum Conditions of Separation Selectivity Based on Molecularly Imprinted Polymers of Bovine Serum Albumin Formed on Surface of Aminosilica氨基硅球表面印迹牛血清白蛋白分离条件的初步探讨
15.Immunocytochemistry and western blotting technology were used to identify the cell phenotype.用蛋白质印迹免疫检测技术和免疫细胞化学技术鉴定细胞表型。
16.The Diagnosis of Bile Duct Carcinoma by Analysis of Sugar Chain of Glycoprotein in Bile with Lectin Dot blotting凝集素点印迹法分析胆汁糖蛋白糖链结构诊断胆管癌
17.Study on Protein-Imprinted Polymeric Microspheres with Pyrrolidone;含吡咯烷酮基团的蛋白质印迹聚合物微球的研究
18.Study on the Recognition of Azo Dye and Protein Moleculer by Molecularly Imprinted Technique;分子印迹技术在偶氮染料和蛋白质分子识别上的应用研究
相关短句/例句
Western blotting蛋白印迹
1.METHODS:PDGFRαand C-kit gene mutations and protein expression in 52 cases of GIST were detected by polymerase chain reaction single- strand conformation polymorphism,immuno- histochemistry andWestern blotting.方法:采用单链象多态性聚合酶链式反应(PCR-SSCP),免疫组化和蛋白印迹(Western blot)方法,检测GIST 52例中PDGFRα和C-kit基因突变及蛋白表达情况。
2.Method:Western blotting technique was used to test 60 serum samples.方法 :用蛋白印迹技术检测与麦芽糖结合蛋白 (MBP)融合的 2种重组梅毒螺旋体蛋白 (rTP47和rTP17)与二期梅毒患者血清的反应性。
3)Western-blot蛋白印迹
1.The levels of bcl-2 and bax expression in cardiomyocytes were measured by RT-PCR andWestern-blot.光镜下观察细胞形态;逆转录-多聚酶链反应和蛋白印迹法检测bc l-2、bax蛋白在心肌细胞中的表达情况。
4)Western blot蛋白质印迹
1.The expression of FATP4 genes in fatty liver rats was examined by semi-quantitative reverse transcription and polymerase chain reaction (RT-PCR) andWestern blotting.方法建立高脂饮食脂肪肝模型 ,用半定量逆转录聚合酶链反应 (reversetranslationpolymerasechainreaction ,RT PCR)与蛋白质印迹 (Westernblot)方法测定脂肪肝肝组织中FATP4表达变化。
5)Western blotting蛋白质印迹
1.The expression of phosphor-p42/44 MAPK was measured byWestern blotting.方法 :DADS处理CNE2细胞 2 4h后 ,荧光显微镜下观察形态学变化及凋亡细胞计数 ,四甲基偶氮唑盐微量酶反应比色法测定细胞活性 ,流式细胞仪检测凋亡细胞 ,蛋白质印迹法检测磷酸化p4 2 4 4MAPK表达。
2.The expression of phospho p38 MAPK was measured byWestern blotting.方法DADS处理CNE2细胞 2 4h后 ,荧光显微镜下观察形态学变化及凋亡细胞计数 ,MTT法测定细胞活性 ,流式细胞仪检测凋亡细胞 ,蛋白质印迹法检测磷酸化p38MAPK表达。
3.UsingWestern blotting technique, the effects of auditory deprivation and tone exposure on the relative expression level (REL) of NMDA receptor subunit NR2B protein of rat auditory cortex during early postnatal days were studied.应用蛋白质印迹检测技术,研究早期听觉剥夺、经验对大鼠听皮层NMDA受体NR2B蛋白表达的影响。
6)Western blotting蛋白印迹法
1.Radioimmunoassay, RT-PCR andWestern blotting techniques were used to evaluate the change of brain COX expression at different time points( before SNI, 1 h, 12 h, 1 d, 3 d, 7 d, 14 d, 30 d and 60 d after SNI); By exploring hot plate test, we observed the reacting time of animals after injection of saline, NS-398, SC-560 and ind.对小鼠施行SNI后,我们采取放射免疫法,RT-PCR法和蛋白印迹法测定术前以及术后1h、12h、1d、3d、7d、14d、30d、60d等不同时间点的脑COX-1和COX-2的表达;采用热板法,分别测定小鼠在各时间点注射生理盐水、NS-398、SC-560或吲哚美辛后的疼痛阈值。
2.Methods: By usingWestern blotting (WB), RPR test and TPPA, serologic antibodies were detected in 67 cases of syphilis, of whom 21 were followed up for 18 to 24 months after treatment.方法:采用蛋白印迹法(Westernblotting,WB)、快速血浆反应素(RPR)试验、梅毒颗粒凝集试验(TPPA)3种方法,对67例梅毒及其中经治疗后随诊18～24个月的21例患者作血清检测。
3.The techniques of diagnosis include ELISA andWestern blotting.细菌病原诊断技术包括酶联免疫吸附技术（ＥＬＩＳＡ）及蛋白印迹法（Ｗｅｓｔｅｒｎｂｌｏｔｉｎｇ）。
延伸阅读
蛋白印迹法分子式：CAS号：性质：一种类似于DNA斑迹法，用于从凝胶中转移蛋白质的技术。转移到膜上的蛋白质用同位素或酶标记过的抗体显示目的带的存在。
