蛋白质印迹法,Western blotting
1)Western blotting蛋白质印迹法
1.), total RNA of livers were extracted and kinetics of the mRNA expression of iNOS were detected by RT-PCR, the protein expression of iNOS was then confirmed byWestern blotting and the distribution of iNOS in the infected liver was determined by immunohistochemical .方法日本血吸虫尾蚴经皮肤感染NMRI小鼠 ,感染后第 0、2 1、2 8、3 8和 45天取其肝组织 ,用逆转录聚合酶链反应 (RT PCR)半定量法检测i NOS转录水平的动态表达 ,用蛋白质印迹法 (Westernblotting)测定表达的蛋白变化 ,并用免疫组化方法确定iNOS在肝组织中的表达定位。
英文短句/例句
1.southwestern blottingsouthwestern印迹法,DNA-蛋白质印迹法
2.Determination on CytochromeC in Various Tissues of Mice by Western-blotting;蛋白质印迹法检测小鼠不同器官中细胞色素C的含量
3.Northwestern blottingNorthwestern印迹(法),RNA-蛋白质印迹(法)
4.protoBlot system[商]蛋白印迹系统[Promega公司生产的蛋白质印迹成套试剂]
5.Study on Protein-Imprinted Soft-wet Gel Polymeric Beads;蛋白质印迹软湿凝胶聚合物微球的研究
6.Bio-imprinting of Protein/DNA Macromolecules and Electro-driven Catalysis of Enzyme;蛋白质/核酸的生物印迹及酶的电驱动催化研究
7.Preparation and Selective Permeation of Protein Molecularly Imprinted Membranes蛋白质分子印迹膜的制备和渗透性研究
8.Detection of E-cadherin expressions in lung cancer tissues by Western blot analysis蛋白质免疫印迹技术检测肺癌组织上皮钙粘蛋白的表达
9.③The changes of BMP2 and osteopontin were detected by immunohistochemistry and Western blotting.③骨形态发生蛋白2与骨桥蛋白检测:采用免疫组织化学染色与蛋白印迹方法。
10.Immunocytochemistry and western blotting technology were used to identify the cell phenotype.用蛋白质印迹免疫检测技术和免疫细胞化学技术鉴定细胞表型。
11.Study on Protein-Imprinted Polymeric Microspheres with Pyrrolidone;含吡咯烷酮基团的蛋白质印迹聚合物微球的研究
12.Study on the Recognition of Azo Dye and Protein Moleculer by Molecularly Imprinted Technique;分子印迹技术在偶氮染料和蛋白质分子识别上的应用研究
13.Construction of Novel Molecularly Imprinted Electrochemical Sensors and Investigation on Direct Electrochemistry of Protein;新型分子印迹电化学传感器的研制及蛋白质直接电化学研究
14.Study on BSA-Imprinted P(AM-NVP) Beads;蛋白质印迹丙烯酰胺—乙烯基吡咯烷酮共聚物微球的研究
15.Preparation of protein molecular imprinted polymer hydrogel by radiation grafting of polypropylene辐射接枝聚丙烯纤维制备蛋白质分子印迹聚合物水凝胶
16.The Diagnosis of Bile Duct Carcinoma by Analysis of Sugar Chain of Glycoprotein in Bile with Lectin Dot blotting凝集素点印迹法分析胆汁糖蛋白糖链结构诊断胆管癌
17.Biological Factors Affecting the Determination of HIV Antibody影响蛋白印迹法检测HIV抗体结果的生物学因素分析
parison of Physical Methods of Protein Extraction in Rabbit Bone Tissue for Western Blot兔骨组织总蛋白的提取和在免疫印迹法中的应用
相关短句/例句
Western blot蛋白质印迹法
1.UseWestern blot to identify the purified protein and vaccinated mice to test the antibody titer by indirect ELISA.然后经柱层析获得纯化蛋白,由蛋白质印迹法验证蛋白的特异性。
2.This result also confirmed by theWestern blot assay using TrpE-gp45 as recombinant antigen,PCR of BIV RT sequence(which is highly conserved among lentiviruses)and sl.当以TrpE-p26融合蛋白做抗原,应用蛋白质印迹法对我国近年进口牛及其后代进行检测时,在所检测的689份样品中,检出阳性样品16份,阳性率为2。
3)western blotting蛋白质印迹(法),Western印迹法
4)Western blotting method蛋白质印迹方法
5)blotting Farwestern印迹法,蛋白质检测(的)蛋白质印迹法
6)Western blot蛋白质印迹
1.The expression of FATP4 genes in fatty liver rats was examined by semi-quantitative reverse transcription and polymerase chain reaction (RT-PCR) andWestern blotting.方法建立高脂饮食脂肪肝模型 ,用半定量逆转录聚合酶链反应 (reversetranslationpolymerasechainreaction ,RT PCR)与蛋白质印迹 (Westernblot)方法测定脂肪肝肝组织中FATP4表达变化。
延伸阅读
蛋白质印迹法分子式:CAS号:性质:一种免疫测定微量蛋白质的技术。样品经SDS-聚丙烯酰胺凝胶电泳后,将分辨的蛋白质转移到硝酸纤维膜上,加入放射标记的抗体一起保温,进行抗原抗体结合反应。然后冲洗去掉未结合的抗体,将薄膜贴在X线底片上,通过放射自显影进行定位和检测。