限制性显示,Restriction display
1)Restriction display限制性显示
1.In order to screen the HIV-1 F subtype gene probes amplified by restriction display, the genechips were prepared by printing the gene fragments onto the slides with printer(PixSys 5500).为筛选限制性显示技术制备的HIV 1F亚型基因探针 ,应用基因芯片打印仪将其有序地打印在玻片上制备基因芯片 。
2.To show the flexible adapter design of restriction display (RD) technique in constructing peptide library, two series of adapters were designed according to vector pET22b in E.为了探讨限制性显示(RD)技术在构建蛋白质多肽文库中灵活的接头设计,分别根据原核表达载体pET22b以及酵母表达载体pNMT-TOPO设计了三套接头,三套接头依次增加一个碱基以保证与之连接的片段总有可能表达正确的开放阅读框。
3.Studies of effect factors on the hybridization of gene expression microarray of K562 cells showed the length of probes prepared by the technology of restriction display PCR was similar,and fit to hybridization of gene chip.通过对K562细胞基因表达谱芯片杂交影响因素的研究表明,用限制性显示技术[1]制备的cDNA探针长度较均一,适合基因芯片杂交;在42℃条件下含甲酰胺的杂交液中杂交16～20h,可保证样品的有效杂交,并表现出很好的杂交特异性;用RD-PCR或线性PCR对少量样品进行扩增,并用荧光(Cy3或Cy5)标记的通用引物对样品进行标记,可提高芯片检测的灵敏度;一次杂交反应总RNA的用量仅需0。
英文短句/例句
1.Application of RD-PCR Technology in the Preparation of HCV Microarray;限制性显示PCR技术在丙型肝炎病毒基因芯片制备中的应用
2.Application of Restriction Display (RD) Technology in HIV Gene-chips for Early Diagnosis of HIV Infection;限制性显示（RD）技术在HIV感染早期诊断基因芯片中的应用
3.Appling RD-PCR Technique to Construct HIV Random Peptide Library;限制性显示技术在HIV随机多肽文库构建中的应用
4.Select Show only to user and administrator to restrict this property to the personal and administrator views of the user profile.选中“仅向用户和管理员显示”可限制该属性仅在用户配置文件的个人视图和管理员视图中显示。
5.All structural materials behave plastically above their elastic range.超过弹性极限时，一切结构材料都显示出塑性。
6.If the number of data rows per page is to be limited, a limit must be specified.如果要限制一页显示的数据行数，则请指定限制值。
7."Turn on option to show only registered rooms in room list""打开选项显示闲聊室列表中受限制的闲聊室"
8.A bounded rationality revealed preference and satisfying decision analysis一类有限理性的显示偏好和满意决策分析
9.Reveal Infinite game save II显示无限游戏存档II
10.Research on Electronic Ink Display Performance by MCU and Manufacture of Display Devices基于单片机控制的电子墨水显示性能研究和显示器件制作
putational Comparison of Revelation Principle Based on Mechanism Truth;基于机制诚实性的显示原理算法比较
12.Learn how to customize the displaying of the property names and descriptions.这个项目演示了如何定制属性名和描述的显示。
13.The display control panel is unable to change the display settings. You may not have appropriate administrative rights to change these settings显示控制面板无法更改显示设置。您可能没有更改这些设置所必需的管理员权限。
14.The screen was not visible due to a limitation of your video card or display monitor. Please try different settings for your display.由于视频卡或显示监视器的限制，看不见屏幕。请为显示试用其他设置。
15.This option limits only the search results displayed under "Suggested Topics."此选项只限制显示在“建议的主题”下面的搜索结果。
16.Can"t not show the result due to resource limitation. Please save the result to file instead.因为资源的限制无法显示结果。请将结果保存在文件中。
17.The traditional idea of serving the people bears a certain degree of limitations in the new era.在新的时代条件下，“为人民服务”的传统观念显示出一定的局限性。
18.The Display Name is the name of the property that is shown whenever the property is shown. It can be changed at any time.“显示名称”是显示属性时所显示的属性名称。您可随时更改“显示名称”。
相关短句/例句
restriction display technology限制性显示技术
1.Virus target sequences were marked byrestriction display technology.方法根据Genebank中病毒基因组序列的保守区域,设计病毒的特异性检测探针,制备病毒检测芯片;利用限制性显示技术标记样品中的病毒靶序列,将标记产物与芯片杂交、清洗、扫描及结果分析。
3)RD PCR限制性显示-PCR
4)Restriction display PCR (RD-PCR)限制性显示(RD)
5)Restriction display PCR限制性显示PCR
6)distinct restriction显性限制
延伸阅读
限制性片段长度多态性分子式：CAS号：性质：又称限制片长多态性。每一种基因型的DNA对某些限制性内切酶有固定的酶切位点，经酶作用后，在凝胶电泳图谱上产生固定的片段图谱。如基因型发生变化，则酶切位点或数目可能有差异，则产生的电泳图谱会有差异。这方法常用于遗传基因型的检查或病理检查。一般来讲高等生物个体都有特定的RFLP。
