聚合酶链式反应-限制性片段长度多态性,PCR-RFLP
1)PCR-RFLP聚合酶链式反应-限制性片段长度多态性
1.MATERIALS AND METHODS: The distribution of the -2518 G/A polymorphism of MCP-1 gene was analyzed from the 92 patients with keloid and 180 unrelated healthy controls with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis and DNA sequence analysis.材料与方法:以92例瘢痕疙瘩患者和180例性别、年龄匹配的正常人为对象进行病例-对照研究,采用聚合酶链式反应-限制性片段长度多态性(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)技术结合DNA测序对MCP-1基因启动子区-2518G/A多态性进行分析。
2)PCR-RFLP聚合酶链反应-限制性片段长度多态性
1.Methods: After gene diagnosis of CSMA for the proband, amniotic fluid of seven pregnancies was collected for fetal DNA extraction and analysis of the survivial motor neuron (SMNt) gene was performed by using polymerase chain reaction with restriction fragment length polymorphism (PCR-RFLP) method.方法:通过应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术,首先对7例家系中的CSMA先证者进行基因诊断,并检测到其运动神经元存活基因(SMNt)第7、8外显子全部缺失,然后抽取孕妇羊水,盐析法提取羊水中的胎儿DNA,运用PCR-RFLP方法对胎儿SMNt基因第7、8外显子进行检测。
2.Methods MTHFR gene C677T porlymorphism was detected in 141 subjects,in- cluding 69 in control (CON) group and 72 in cerebral infarction (CI) group byPCR-RFLP technique.方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测72例脑梗死患者和69例对照者的MTHFR基因C677T多态性分布,并进行对比分析。
3.Methods:β-Fibrinogen gene G/A-455 polymorphisms have been detected usingPCR-RFLP technique in 72 patients with cerebral infarction(CI) and in 69 healthy controls(CON).方法:应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,检测脑梗死组(CI组)72例和对照组69例-βFg-455G/A基因的多态性,并测定其血浆纤维蛋白原(Fg)的含量。
英文短句/例句
1.The distribution of polymorphism at restriction enzyme site-48 A/ G was analyzed with the method of polymerase chain reaction-restriction fragment length polymorphism( PCR-RFLP).用聚合酶链反应-限制性片段长度多态性方法,分析限制性酶切位点-48A/的多态分布。
2.Identification of 60 strains of clinical isolated Microsporum canis with restriction fragment length polymorphism60株临床分离犬小孢子菌的聚合酶链反应-限制性片段长度多态性鉴定
3.restriction fragment length polymorphism限制性片段长度多态性
4.Analysis of YMDD Mutants Of The Viral Polymerase in Hepatitis B Virus Infected Patients With Iamivudine Therapy By Means Of Restriction Fragment Length Polymorphism(RFLP)Of The PCR Product;拉米夫定抗HBV治疗中聚合酶YMDD变异的PCR-限制性片段长度多态性分析
5.The Establishment of An Amplified Fragment Length Polymorphism Reaction System with Gastrodia elata Blume天麻扩增酶切片段长度多态性反应体系的建立
6.Optimation of amplification length polymorphism (AFLP) reaction system with Gastrodia elata Blume天麻扩增酶切片段长度多态性反应体系的优化
7.Analysis of MDR1 gene-3435C/T polymorphisms by allele-specific polymerase chain reaction等位基因特异性聚合酶链反应检验多药耐药基因的多态性
8.fragment length polymorphism片段长度多态性，片长多态性
9.RESTRICTION FRAGMENT LENGTH POLYMORPHISM OF Bam HI AND Rsa I LOCI IN视网膜母细胞瘤基因的BamHI和RsaⅠ限制性片段长度多态性
10.Malassezia Species Constitution in Normal Human Cerumen and Research for Its Restriction Fragment Length Polymorphism;正常人盯聍中马拉色菌菌种构成及限制性片段长度多态性分析
11.H-ras Gene Mutation Detection of Gastric Cancer by Restriction Fragment Length Polymorphism with Capillary Electrophoresis毛细管电泳-限制性片段长度多态性分析检测胃癌H-ras基因点突变
12.Detection of Minimal Residual Leukemia Cells in Acute Myeloid Leukemia by Polymerase Chain Reaction Single Strand Conformational Polymorphism;应用聚合酶链反应单链构象多态性分析技术检测微量残留急性髓细胞白血病的研究
13.Detection of Streptomycin Resistant Gene of Mycobacterium Tuberculosis by PCR and Single-Strand Conformation Polymorphism Analysis聚合酶链反应—单链构象多态性技术鉴别结核分支杆菌链霉素耐药基因
14.Preparation of Single-Stranded DNA Template for Pyrosequencing by Linear-after-the-Exponential-Polymerase Chain Reaction指数线性聚合酶链式反应法制备焦磷酸测序反应的单链模板
15.Rapid Detection of Gene Mutation in Multiple-drug Resistant Mycobacterium Tuberculosis by PCR and Single-strand Conformation Polymorphism Analysis;聚合酶链反应—单链构象多态性技术快速检测耐多药结核分枝杆菌突变基因
16.Detection of Isoniazid Resistant Gene of Mycobacterium Tuberculosis by PCR and Single-strand Conformation Polymorphism Analysis聚合酶链反应—单链构象多态性技术鉴别结核分支杆菌异烟肼耐药基因
17.Detection of Rifampin Resistant Gene of Mycobacterium Tuberculosis by PCR and Sigle-strand Conformation Polymorphism Analysis;聚合酶链反应—单链构象多态性技术鉴别结核分支杆菌利福平耐药基因
18.Amplified Fragment Length Polymorphism Analysis ofApoB Locus in Tissue andIts Application in Forensic Science;组织ApoB位点扩增片段长度多态性分析及其应用
相关短句/例句
PCR-RFLP聚合酶链反应-限制性片段长度多态性
1.Methods: After gene diagnosis of CSMA for the proband, amniotic fluid of seven pregnancies was collected for fetal DNA extraction and analysis of the survivial motor neuron (SMNt) gene was performed by using polymerase chain reaction with restriction fragment length polymorphism (PCR-RFLP) method.方法:通过应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术,首先对7例家系中的CSMA先证者进行基因诊断,并检测到其运动神经元存活基因(SMNt)第7、8外显子全部缺失,然后抽取孕妇羊水,盐析法提取羊水中的胎儿DNA,运用PCR-RFLP方法对胎儿SMNt基因第7、8外显子进行检测。
2.Methods MTHFR gene C677T porlymorphism was detected in 141 subjects,in- cluding 69 in control (CON) group and 72 in cerebral infarction (CI) group byPCR-RFLP technique.方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测72例脑梗死患者和69例对照者的MTHFR基因C677T多态性分布,并进行对比分析。
3.Methods:β-Fibrinogen gene G/A-455 polymorphisms have been detected usingPCR-RFLP technique in 72 patients with cerebral infarction(CI) and in 69 healthy controls(CON).方法:应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,检测脑梗死组(CI组)72例和对照组69例-βFg-455G/A基因的多态性,并测定其血浆纤维蛋白原(Fg)的含量。
3)polymerase chain reaction-restriction fragment length polymorphism聚合酶链反应-限制性片段长度多态性
1.Methods PCR primers were designed by create restriction site(CRS) method, thenpolymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was adopted to identify four SNPs in MGMT gene.方法应用碱基错配创造酶切位点原理设计引物,通过聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术判断MGMT基因四个SNP位点多态性。
2.Objective Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) assay was applied to molecular typing of resistance-associated esterase genes of Culex pipiens complex.目的应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)检测法,建立尖音库蚊抗性酯酶等位基因分型方法。
3.Methods apoE genotype was assayed bypolymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).方法采用聚合酶链反应-限制性片段长度多态性和双向电泳-免疫印迹检测法,分析103例型高脂血症患者和146名血脂正常者的apoE基因型、HDL各亚类组成及相对含量。
4)polymerase chain reaction restriction fragment length polymorphism聚合酶链反应-限制性片段长度多态性
1.Methods APOE genotype was assayed bypolymerase chain reaction restriction fragment length polymorphism (PCR RFLP).方法应用聚合酶链反应-限制性片段长度多态性和双向电泳-免疫印迹检测法,分析112例高脂血症患者和73名正常对照者APOE基因型、HDL各亚类组成及相对含量。
5)polymerase chain reaction/restriction fragment length polymorphism聚合酶链反应-限制性片段长度多态性
6)PCR-RFL聚合酶链式反应-限制性片段长度多态性分析
延伸阅读
限制性片段长度多态性分子式：CAS号：性质：又称限制片长多态性。每一种基因型的DNA对某些限制性内切酶有固定的酶切位点，经酶作用后，在凝胶电泳图谱上产生固定的片段图谱。如基因型发生变化，则酶切位点或数目可能有差异，则产生的电泳图谱会有差异。这方法常用于遗传基因型的检查或病理检查。一般来讲高等生物个体都有特定的RFLP。
