细胞内游离钙浓度,intracellular free calcium concentration
1)intracellular free calcium concentration细胞内游离钙浓度
1.In this present study, the effects of ET 1 onintracellular free calcium concentration ([Ca 2+ ] i) and the underlying mechanisms were investigated in cultured neonatal rat myocardail cells loaded with fura 2/AM.实验用培养新生ＳＤ 大鼠心室肌细胞， 以Ｆｕｒａ２/ＡＭ 荧光指示剂负载检测心肌细胞内游离钙浓度（［Ｃａ２ ＋ ］ｉ） 的变化， 探讨内皮素１（ＥＴ１） 对［Ｃａ２ ＋ ］ｉ 的作用及其机制。
2.For mechanism studies,theintracellular free calcium concentration(i) in cultured cardiomyocy.AN-FmRNA的表达用Real-time PCR检测;用Fura-2/AM负载的培养心肌细胞检测细胞内游离钙浓度([Ca2+]i);一氧化氮试剂盒测定细胞上清液中一氧化氮(nitric oxide,NO)代谢物的含量,以探讨Rg1可能的作用机制。
英文短句/例句
1.Meanwhile the intracellular free calcium concentration was increased.细胞凋亡的同时细胞内游离钙浓度升高。
2.Effect of theaflavin on intracellular free calcium concentration in rat ventricular myocytes茶黄素对大鼠心室肌细胞内游离钙浓度的影响
3.Effects of 8-Methoxypsoralen on intracellular [Ca~(2+)]i and cytoskeleton actin organization in human melanocytes in vitro8-甲氧沙林对黑素细胞内游离钙浓度及细胞骨架蛋白形成的影响
4.Experimental Research on the Effect of Cyclovirbuxine-D on Intracellular Free Ca~(2+) Concentration in Rat Acute Isolated Cardiomyocytes环维黄杨星D对急性分离大鼠心肌细胞内游离钙浓度的影响
5.STUDY ON THE EFFECTS OF ROTENONE ON THE CONCENTRATION OF INTRACELLULAR FREE CALCIUM IN ASTROCYTE鱼藤酮对星形胶质细胞内游离钙浓度的影响及其机制
6.Study of theaflavin on reducing intracellular free calcium concentration in ventricular myocytes in rats with ischemia/reperfusion injury茶黄素降低缺血再灌注损伤大鼠心室肌细胞内游离钙浓度的研究
7.Protective Effects of Cyclovirobuxine D exposed to Hypoxia-reoxygenation Injury and Experimental Research of Cyclovirbuxine D on Intracellular Free Ca~(2+) Concentration on rat Cardiomyocytes环维黄杨星D对心肌细胞缺氧/复氧损伤的保护作用和对心肌细胞内游离钙浓度的影响
8.Effects of fistular onion bulb extract preconditioning on ischemia-reperfusion injury in vitro and intracellular free Ca~(2+) increase in hearts of rats葱白提取物预处理对大鼠离体心脏缺血再灌注损伤和心肌细胞内游离钙浓度的影响
9.Effect of Hypertonic Solution on Free Calcium in Endothelial Cells Suffering from Hypoxia/reoxygenation;高渗液对缺氧/复氧内皮细胞内游离钙离子浓度的影响
10.Effect of hypertonic solution on free calcium in human umbilical vein endothelial cells suffering from hypoxia/reoxygenation高渗液对缺氧/复氧人脐静脉内皮细胞内游离钙离子浓度的影响
11.Effects of Arecolinehydrobromide on Intracellular Free Ca～(2+) of Colonic Smooth Muscle Cells;槟榔碱对结肠平滑肌细胞内游离钙离子浓度的影响
12.Effects of Androgen on Intracellular Free Calcium Concentration of Prostate Cancer Cells and Its Underlying Mechanism;雄激素对前列腺癌细胞内游离钙离子浓度的影响及其机制探讨
13.Study on Intracellular Ca～(2+) Concentration with Ratio Fluorescence Image Master;比率荧光光谱仪测量细胞内游离钙离子浓度的研究
14.Influences and Mechanisms of Advanced Glycation End Products on Cytosolic Free Calcium Concentration in Cultured Neonatal Rat Cardiac Myocytes.;晚期糖基化终产物对心肌细胞胞内游离钙浓度的影响及其机制
15.The concentration ofcalcium was assessed by ACAS (adherent cell analysis and sortingcytorneter).用粘附式细胞仪测定细胞内钙离子浓度；
16.Studies on the Heterogeneity of Basal Intracellular Calcium Concentration and its Relations to the Reactivity of Mouse Peritoneal Macrophages;小鼠腹腔巨噬细胞内基态游离钙离子浓度的不均一性及其和细胞反应性的关系
17.Effect of CTPZ on Cytosolic Free Calcium Concentration in Smooth Muscle Cells川芎嗪甲酸对平滑肌细胞游离钙浓度的影响
18.MAIN OUTCOME MEASURES① Changes of neuronal cytoplasmic free calcium concentration.主要观察指标:①经元胞浆内游离钙浓度变化。
相关短句/例句
[Ca~ 2+ ]_ i细胞内游离钙离子浓度
1.Cytotoxicity was evaluated by MTT assay, intracellular Rh123 retention was measured by flow cytometry, and cellular [Ca~ 2+ ]_ i was measured b.方法:将不同浓度的洛美利嗪与人红白血病细胞系K562及其耐药细胞系K562/A02(耐阿霉素)共孵育24、48或72小时,然后分别向细胞中加入阿霉素,采用MTT法检测细胞毒作用;以流式细胞术测定两种细胞系内罗丹明123的潴留以反映P-糖蛋白的外排功能;利用Fluo-3/AM检测细胞内游离钙离子浓度。
3)intracellular calcium concentration胞内游离钙浓度
1.By using Fura-2 microfluorometry technique, the effects of toosendanin on theintracellular calcium concentration([Ca 2+] i) in single rat chromaffin cell were studied.在单个大鼠肾上腺嗜铬细胞上 ,采用Fura 2显微荧光测量技术 ,测量了川楝素对胞内游离钙浓度([Ca2 +]i)的影响。
2.By using microflurescent calcium measurement technique, the effects of extract of Zhi Bo Di Huang Wan on theintracellular calcium concentration ([Ca 2+ ] i) of single rat Chromaffin cell were studied.运用Fura 2显微荧光测量技术 ,在单个大鼠肾上腺嗜铬细胞上 ,测量中药知柏地黄丸浸液对胞内游离钙浓度 ([Ca2 + ]i)的作用 。
4)intracellular free calcium胞内游离钙浓度
1.Effects of flavonoids of Hippophae rhamnoides L onintracellular free calcium concentration in cultured rabbit aortic smooth muscle cells;醋柳黄酮对血管平滑肌细胞胞内游离钙浓度的影响
2.Aim: To explore the effects of quercetin (Que) onintracellular free calcium ([Ca~(2+)] i) in vascular smooth muscle cells.目的：探讨槲皮素（Ｑｕｅ）对血管平滑肌细胞胞内游离钙浓度（［Ｃａ~（２＋）］ｉ）的影响。
5)Cytosoic free calcium concentration胞浆内游离钙离子浓度
6)intracellular calcium细胞内钙离子浓度
1.To study the prevention of dauricine (Dau) on bradykinin (BK) induced alteration ofintracellular calcium homeostasis and tau phosphorylation, fluorescence spectrophotometer with dual excitation was utilized to measure theintracellular calcium concentration ([Ca2+]i), MTT to detect cell viability and immuncytochemistry to examine tau phosphorylation.为研究蝙蝠葛碱(dauricine,Dau)拮抗缓激肽(bradykinin,BK)诱导的Alzheimer样钙稳态失衡及细胞骨架蛋白异常磷酸化的作用,采用双波长荧光分光光度计测定细胞内钙离子浓度([Ca2+]i),用MTT法检测细胞代谢水平,用免疫组织化学方法观察tau蛋白表达和磷酸化。
延伸阅读
细胞内钙稳态失调在细胞功能的调节中，ca2+可作为第二信使起着信号传导的关键作用，同时ca2+ 也是多种参与蛋白质、磷脂和核酸分解的酶的激活分子之一。正常情况下，细胞内钙稳态是由质膜ca2+转位酶和细胞内钙池系统共同操纵控制的。细胞损害时，这一操纵过程紊乱可导致ca2+内流增加，ca2+从细胞内储存部位释放与/或通过质膜逐出抑制，从而导致细胞内ca2+浓度不可控制的持续增加，细胞内ca2+ 浓度持续高于生理水平以上必然导致维持细胞结构和功能的重要大分子难以控制的破坏。而且这种持续增加将会完全破坏正常生命活动所必需的由激素和生长因子刺激而产生的短暂的ca2+浓度瞬变，危及线粒体功能和细胞骨架结构，最终激活不可逆的细胞内成分的分解代谢过程。毒物可在不同水平上干扰细胞信号的传递，导致细胞内ca2+ 对激素及生长因子的正常反应的丧失。另外，钙信号系统的异常活化也是毒物引起细胞死亡的一个重要机制。当前，细胞内钙稳态失调是细胞损害与机制研究方面最为热门的话题，大量证据表明，细胞钙的持续增高可能活化各种不同组织和细胞的毒性机理，因而曾被称为“细胞死亡的最终共同途径”。
