SYBR greenⅠ,SYBR green Ⅰ
1)SYBR green ⅠSYBR greenⅠ
1.A rapid and sensitive Real-time PCR assay coupled with SYBR Green Ⅰchemistry was developed for the quantitative detection of Turbot reddish body iridovirus (TRBIV) isolated from farmed turbot (Scophthalmus maximus).研究利用TRBIV主要衣壳蛋白基因序列设计的1对引物,结合内嵌式核酸染料SYBR GreenⅠ,建立了TRBIV特异的Real-timePCR检测方法。
2)SYBR GreenⅠfluoresence quantitative reverse transcriptase-polymerase chain reactionSYBR GreenⅠ荧光定量PCR
1.SYBR GreenⅠfluoresence quantitative reverse transcriptase-polymerase chain reaction for measurement of IL-2 and IL-10 gene expressions in patients with renal transplantation;方法采用SYBR GreenⅠ荧光定量PCR对29例肾移植患者和10例常规手术患者术前、术后3和7天外周血淋巴细胞IL-2和IL-10 mRNA表达进行检测。
3)SYBR Green ⅠSYBR GreenⅠ荧光染料
4)SYBR-GreenⅠ real-time PCRSYBR-GreenⅠ实时荧光PCR
1.TheSYBR-GreenⅠ real-time PCR was established by the positive quality control.利用阳性质控品建立SYBR-GreenⅠ实时荧光PCR方法,对反应体系进行优化,进行特异性和敏感性分析,并制作标准曲线。
英文短句/例句
1.DETECTION OF INFECTIOUS SALMON ANAEMIA VIRUS WITH SYBR-GREEN I REAL-TIME PCR ASSAY;SYBR-GreenⅠ实时荧光PCR检测传染性鲑鱼贫血病
2.A Sensitive and Specific Method of Real-time Fluorescence Quantitative Polymerase Chain Reaction Using SYBR GreenⅠ for Detection of c-myc GeneSYBR GreenⅠ实时荧光定量PCR检测人c-myc基因方法的建立
3.The study of SYBR GreenⅠ real-time PCR assay and detection of genetically modified foodSYBR GreenⅠ实时荧光PCR检测食品中转基因成分方法的初步建立
4.Development of Melting Curve-based SYBR Green Ⅰ PCR for Detecting Two Pathogenic Bacteria应用SYBR GreenⅠ荧光PCR熔解曲线同时检测两种致病菌
5.Development of SYBR-GreenⅠ real-time quantitative PCR assay for detection of pseudorabies virus猪伪狂犬病毒SYBR-GreenⅠ实时定量PCR检测方法的建立
6.Development of SYBR Green Ⅰ real-time PCR assay for detection of porcine parvovirus猪细小病毒SYBR GreenⅠ实时定量PCR检测方法的建立
7.Development of SYBR Green Ⅰ Fluorescence Quantitative RT-PCR Assay for Detection of Porcine Reproductive and Respiratory Syndrome Virus猪繁殖与呼吸综合征病毒SYBR GreenⅠ荧光定量RT-PCR方法的建立
8.Detection of Transgenic Components in Soybean Products by SYBR Green Real-Time PCRSYBR Green实时荧光定量PCR检测大豆转基因成分
9.Establishment of a Differential Method of Highly Pathogenic Strain and Classical Strain of PRRSV by SYBR Green-Ⅰ Real-time PCR AssayPRRSV高致病株和经典株SYBR Green-Ⅰ实时荧光PCR鉴别方法的建立
10.Development of SYBR Greenireal-time Quantitative PCR Assay to Detect Porcine Reproductive and Respiratory Syndrome VirusSYBR Green Ⅰ实时荧光定量PCR检测猪繁殖与呼吸综合征病毒
11.Development and Application of SYBR Green I Real-Time PCR Technique for Detecting Hog Cholera Virus猪瘟病毒SYBR Green I实时荧光定量PCR检测方法的建立和初步应用
12.Development of a SYBR-Green 1 real-time fluorescent quantitative PCR method for detection of type 2 porcine circovirus猪圆环病毒2型SYBR-Green 1实时荧光定量PCR检测方法的建立
13.Establishment and application of real-time PCR assay based on SYBR green for detection and quantization of Cryptosporidium parvum基于SYBR Green检测微小隐孢子虫的实时荧光定量PCR方法的建立及应用
14.Establishment and Application of SYBR-Green1 Fluorescent Quantitative PCR Method for Detecting Toxoplasma Gondii B1 Gene;SYBR-Green1荧光定量PCR检测弓形虫B1基因实验方法的建立及应用
15.Development of a real-time PCR assay based on SYBR GreenⅠ for detection of porcine encephalomyocarditis virus猪脑心肌炎病毒SYBR GreenⅠ real-time PCR检测方法的建立
16.The Development of Real Time PCR Assay for Detection of Mycoplasma Hyopneumoniae;猪肺炎支原体SYBR GREEN荧光定量PCR检测技术研究
17.Detection of Porcine Circovirus-like Agent P1 Using SYBR Green Ⅰ Fluorescent Quantitative PCR AssaySYBR Green Ⅰ荧光定量PCR检测类猪圆环病毒因子P1
18.DETECTION OF CANINE TUBERCULOSIS BY USING SYBR GREEN QUANTITATIVE PCR ASSAY犬结核SYBR Green荧光定量PCR检测方法的建立
相关短句/例句
SYBR GreenⅠfluoresence quantitative reverse transcriptase-polymerase chain reactionSYBR GreenⅠ荧光定量PCR
1.SYBR GreenⅠfluoresence quantitative reverse transcriptase-polymerase chain reaction for measurement of IL-2 and IL-10 gene expressions in patients with renal transplantation;方法采用SYBR GreenⅠ荧光定量PCR对29例肾移植患者和10例常规手术患者术前、术后3和7天外周血淋巴细胞IL-2和IL-10 mRNA表达进行检测。
3)SYBR Green ⅠSYBR GreenⅠ荧光染料
4)SYBR-GreenⅠ real-time PCRSYBR-GreenⅠ实时荧光PCR
1.TheSYBR-GreenⅠ real-time PCR was established by the positive quality control.利用阳性质控品建立SYBR-GreenⅠ实时荧光PCR方法,对反应体系进行优化,进行特异性和敏感性分析,并制作标准曲线。
5)SYBR-GreenⅠReal time RT-PCRSYBR-GreenⅠ荧光定量RT-PCR
6)Taq manSYBR
1.The HBVDNA in culture media were prepared with three methods for isolation and amplified byTaq man and SYBR real-time quatitiative PCR.方法分别采用3种方法抽提HBVDNA,抽提物分别采用Taqman荧光定量、SYBR荧光定量PCR技术检测其滴度。
延伸阅读
Malachite Green Crystals分子式：2(C23H25N2)·3C2H2O4分子量：929.05CAS号：569-64-2性质：绿色闪光结晶，溶于冷水和热水呈蓝绿色，易溶于酒精，也呈蓝绿色。遇浓硫酸呈黄色，稀释后呈暗橙色；其水溶液加氢氧化钠形成微带绿光的白色沉淀。属三苯甲烷系碱性染料。制备方法：由N,N-二甲基苯胺与苯甲醛在硫酸存在下进行缩合，再用二氧化铅和盐酸进行氧化，然后用硫酸钠脱铅，用碳酸钠中和，然后经草酸溶解、结晶、过滤干燥即得成品。原料消耗(kg/t)N,N-二甲苯胺 780盐酸（31%）1250尿素 6 氢氧化钠（100%）350苯甲醛340次氯酸钠4820乙酸铅（98%） 215元明粉（98%） 570草酸（98%）520精盐1900纯碱（98%）290乳化剂OP0.7硫酸（100%） 305用途：用于麻、蚕丝、腈纶织物和草制品以及竹、木等的染色。还可制成各种色淀及颜料。
