表达载体,expression vector
1)expression vector表达载体
1.Construction of shRNAexpression vector pWH1 and its function in silencing the HIF1 gene;shRNA表达载体pWH1的构建及用于HIF1基因的沉默
2.Construction and identification of siRNAexpression vector targeting for Rab9 GTPase;靶向Rab9 GTPase siRNA表达载体的构建和鉴定
3.Construction of Saccharopolyspora erythraeaexpression vector pZW;红色糖多孢菌表达载体pZW的构建
英文短句/例句
1.Construction of Recombinant Plasmid pEGFP-VEGF165 in vitro and Expression in 293T CellsVEGF165真核表达载体的构建与体外表达
2.Construction and expression in vivo of eukaryotic expression vector pcDNA3-CTLA4lg真核表达载体pcDNA3-CTLA4lg的构建及体内表达
3.Construction of Prokaryotic Expression Vector of Human B7h Gene and Its Expression;人B7h基因原核表达载体的构建和表达
4.The Construction and Expression of Lentivirus-Mash1 Expressing Vector;重组Mash1慢病毒表达载体的构建及表达
5.Construction and Expression of hTERT in Eucaryotic Expression Vector;hTERT真核表达载体的构建与表达
6.Expression of TNFR1-IgG4 and EGFP-IgG Eukaryotic Expression Vector;TNFR1-IgG4与EGFP-IgG4真核表达载体的表达
7.The Constructing and Expression of Recombinant Human Fibroblast Growth Fator-8a High Level Expressed Vector;rhFGF8a高效表达载体的构建及表达
8.Construction of eukaryotic expression vector of human miR-34a and it"s expression人miR-34a真核表达载体的构建及其表达
9.Construction and expression of eukaryotic expression vector of human SYNOVIOLIN gene人SYNOVIOLIN基因真核表达载体的构建及表达
10.Construction of eukaryotic expression vector of MyD88 gene and detection of its expressionMyD88基因真核表达载体的构建及表达
11.Construction and expression of the recombinant prokaryotic expression vector with hIL-24重组hIL-24原核表达载体的构建及表达
12.Construction and expression of HBV preS1 gene in yeastHBV preS1基因酵母表达载体的构建及表达
13.Construction of eukaryotic expression vector of siRNA-inhibited CDC25A expression抑制CDC25A表达的siRNA真核表达载体的构建
14.An Expression Vector for Efficient Expression of Soluble Recombinant Proteins--pHisSUMO高效表达可溶性重组蛋白表达载体——pHisSUMO
15.Construction of an eukaryotic expression vector encoding adipophilin and its expression in THP-1 cellsAdipophilin真核表达载体的构建与表达分析
16.Construction of HA-GILZ eukaryotic expression vector and its identified expression in cell lineGILZ真核表达载体的构建及其表达定位
17.Construction and expression of gp350 eukaryotic expression vectorgp350胞外段基因表达载体的构建与表达
18.Construction of a Human Adiponectin Expression Vector and Its Expression in Vitro;脂联素基因表达载体的构建及体外表达研究
相关短句/例句
Expression Vectors表达载体
1.Construction and Functional Analysis of Short Interfering RNAExpression Vectors of Targeting Gene Apolipoprotein M;靶向载脂蛋白M基因小干扰RNA表达载体构建及其功能
2.Construction and application of an expression vectors with combined T7 and PR promoters for flexible induced expression of cloned DNA in E.coli;利用T7和PR双启动子的新型表达载体的构建及其应用
3.This is clearly due to the fact that all the signals contained in lactoprotein genes have not yet been identified,and the mammary-gland expression vectors are not constructed in an appropriate manner.利用转基因动物乳腺生物反应器生产重组蛋白是一项极具潜力和开发价值的生物技术,但成功的例子并不多,主要是由于乳蛋白的表达调控机制还未完全阐明,构建的表达载体不足以克服位置效应的影响,使得外源基因的表达难以预期。
3)Expressing vector表达载体
1.Plant expressing vector construction of MAPK gene in Dunaliella salina and transformation to tobacco;杜氏盐藻MAPK基因植物表达载体的构建及对烟草的转化
2.The construction of E3region expressing vector of CAV-2;犬2型腺病毒E3区表达载体的构建
3.The identifications of PCR, enzymical degestion and sequencing were carried out, and the expressing vector of pG-Lfn was successfully constructed.coli JM-109,并进行PCR鉴定、酶切鉴定及测序,成功构建了Lactoferricin基因表达载体pG-Lfn。
4)vector[英]["vekt?(r)][美]["v?kt?]表达载体
1.Construct of Gastrin-shRNA Vector and Its Effects on Gastric Cancer Cell Line BGC-823;胃泌素-shRNA表达载体的构建及其对胃癌细胞系BGC-823的效应
2.In order to construct a universal mammary gland expressionvector, sheep beta lactoglobulin (BLG) gene promoter, 5′ and 3′ region of 5 kb and 4.２ｋｂ构建乳腺表达载体。
3.This system is derived from commercially availablevector pETlla (Novagen) by 1) changing promotor T7 to SP6; 2) introducing 6xHis tag at N-terminal of open reading frame and 3) inserting a marker gene of RNA Polymerase which is induced by L-arabinose and thus to work as a protein expression control.本研究旨在建立高效表达目的蛋白的原核表达载体系统pESP15b/pARA-SP6。
5)eukaryotic expression vector表达载体
1.Construction of siRNAeukaryotic expression vectors of PEG10 gene and their apoptosis-inducing effect on transfected HepG2 cells;PEG10基因siRNA真核表达载体的构建及其对肝癌HepG2细胞凋亡的影响
2.To construct theeukaryotic expression vector of pCEP4-CaBP-D28k,a pair of primers was designed according to chicken calbindin-D28k(CaBP-D28k) gene sequence published in GenBank.为构建pCEP4-CaBP-D28k真核表达载体,根据GenBank已发表钙结合蛋白D28k(calbindin-D28 k,CaBP-D28 k)设计1对引物,利用RT-PCR方法获得新扬州鸡CaBP-D28 k基因序列,克隆入真核表达载体pCEP4,经PEI介导转入非洲绿猴转化肾细胞(COS-1)中,并以间接免疫荧光试验(IFA)检测CaBP-D28 k表达状况。
6)Express vector载体表达
延伸阅读
表达谱差异分析表达谱差异分析(differential expression profiling)主要包括基因表达谱(gene expression profiling) 和蛋白质表达谱(protein expression profiling) 。大规模表达谱分析已经成为认识疾病分子机制的有利方法,在癌症研究等方面取得了一定的进展。成功的表达谱分析基于实验及其过程分析的有机结合。实验过程从关注的疾病开始,首先收集大量的疾病相关组织样本,样本数量可从10 多个到数百个,但必须足以对每一组织类型及个体差异进行比较分析,而且许多情况下不能仅简单地分为正常和疾病组织。例如,在对糖尿病的研究中,所收集的样本来自健康人、胰岛素耐受和糖尿病病人的不同试验阶段,如胰岛素治疗前后。样品还应包括其他器官的取材,以便进行基因表达的组织分布研究。为了便于对后来的实验数据进行分析管理,需采集并储存所有的组织样本和临床参数。接下来进行组织样本的处理,利用生物芯片(寡核苷酸芯片、cdna 芯片或全基因组芯片) 进行表达谱测定,并进行生物信息学分析。通常,表达谱的分析结果需进一步的实验加以证实。定量rt2pcr 是最灵敏的确证方法,该方法还可以将确证实验的范围扩大到原测组织以外的更广泛的组织和组织类型,揭示基因表达的组织分布情况。确证实验揭示了疾病相关基因。据此,可以进行进一步研究,探索这些基因的功能,开发新的治疗手段。例如,对于正常和疾病组织中表达有显著性变化的基因,可以进行新治疗靶点的鉴定和确定研究,或利用实验和分析工具研究分析其功能;对于疾病组织中活性升高的酶,可以当作前药活化酶进行鉴定研究。典型的表达谱能够显示疾病过程中有大量的已知基因表达的改变,而许多已知基因的代谢通路、表达产物酶学分类和蛋白质功能业已发表,将两者对照分析,可以鉴定出酶活性,选择其中可能成为前药活化酶的部分进行进一步研究;对于疾病特异的蛋白质,可以进行抗原表型分析,决定疫苗的开发策略。寻找差异表达基因的方法除芯片技术外,一些新的检测方法如差异显示pcr ( differential display pcr, dd－pcr) 、消减杂交( suppression subreactive hy bridization , ssh) 等也相继得到结合应用。
